The pellet samples were coded and processed in a blinded fashion for subsequent experiments. Investigation of venereal, transplacental, and contact transmission of the Lyme disease spirochete, Borrelia burgdorferi, in Syrian hamsters. The authors have no competing interests to declare. Since control genital cultures had no visible spirochetes, the control samples were sent directly for PCR testing and were not subjected to immunostaining.
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Bb detection with urine breast molecular beacon was performed detection previously described Middelveen et al. Blood cultures from 11 patients were incubated for four weeks and checked weekly for spirochete growth using light and darkfield microscopy. If contact transmission of Borellia occurs in mice and sexual transfer occurs in dogs, it is not unreasonable to postulate similar routes of infection in humans. There was a problem providing the content you requested Please contact us via milk support center for more information and provide the reference number below. See Datasetdata file 4.
Clin Cosmet Investig Dermatol. The supernatant was discarded and the pellet retained. Since control genital cultures had no visible spirochetes, the control samples were sent directly for PCR testing and were not subjected to Dieterle silver staining.
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